liver cancer

Abstract: The purpose of the use of rotating cell culture system (rotating cell culture system, RCCS) simulated microgravity environment, the establishment of pancreatic cancer liver metastasis in vitro model. Methods human liver tissue and human pancreatic cancer cell line BxPC-3 at rotating cell culture System mixed culture, in the rated time points from the liver tissue send pathology, HE staining of liver tissue structure and pancreatic cancer cells in the liver tissue in growth. result of a mixed culture, Heaven and the surrounding liver tissue within a lumen pancreatic cancer cell adhesion and aggregation; that the first three days of liver tissue in cancer cells scattered infiltration first seven days that small metastases formation of mixed culture on the 10th day of primary liver metastases of pancreatic cancer, liver cell morphology and structure can be Hepatic Lobules remain intact, and that cancer tissue formation. conclusions rotary cell culture system using simulated microgravity environment, constructed in vitro model of liver metastasis of pancreatic cancer, pancreatic cancer liver metastases can be used to study the process and mechanisms for the study of pancreatic cancer cells and liver tissue the relationship between the new method.